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Cell culture

UI or IU units for enzymes

John Andrews Friday 8th August 2003

What's UI stand for? UI stands for Unit International and is the established usage in most of the world excluding North America, where the alternative units of IU (international unit) are used. The units are however interchangable. What are they? UI refers to the activity of a substance. The substance is typically a vitamin or a drug, and the activity refers to the actual effectiveness of the substance. Each substance has an internationally agreed biological effect which relates to a dose of 1 …

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Guide to Cell Counting

V F Murphy< a> Tuesday 5th August 2003

So, you've a lot of cells and you want to know just how many? Cell counting is a key skill and it is crucial to use the correct proceedures.

In this tutorial we assume that you have obtained your cells in a suspension within an unknown volume of some fluid. If this is not the case, it is advantageous if the cells are manipulated to this stage before commencing counting. Resources Needed Hemacytometer - including cover slip

Microscope - many types are suitable

Handheld counter - of the click …

Sources of Cells

James Anderson Thursday 5th June 2003

The source of cells to be used within studies are divided into two main groups depending upon the origin. The first group, Primary Culture, refers to those cells which are directly obtained from tissue, whereas Non-primary (Secondary, Tertiary, etc.) sourced cells are those obtained from existing isolated populations. Primary Culture Sources The primary sourced cells must be isolated from the tissue in some way. Most tissues surround cells with a biologically fabricated matrix which makes it har…

Introducing Cell Culture

V F Murphy< a> Thursday 5th June 2003

Cell culture Cell culture in essence is the removal of cells from their normal physiological in vivo environment and sustaining viability or promoting proliferation. The On-line Medical Dictionary defines cell culture as a general term referring to the maintenance of cell strains or lines in the laboratory. Cell culture - a general term referring to the maintenance of cell strains or lines in the laboratory On-line Medical Dictionary

Why Cell Culture Cell culture enables many studies that wo…